Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 879, Issue 25, Pages 2601-2610Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2011.07.018
Keywords
Antibiotics; Milk; LC-MS/MS; UPLC-MS/MS; Matrix effect; Validation
Funding
- Ministerio de Educacion y Ciencia of the Spanish Government [CTQ2010-19044/BQU]
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The aim of this study was to develop and validate an analytical method to simultaneously determine European Union-regulated beta-lactams (penicillins and cephalosporins) and quinolones in cow milk. The procedure involves a new solid phase extraction (SPE) to clean-up and pre-concentrate the three series of antibiotics before analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). LC-MS/MS and UPLC-MS/MS techniques were also compared. The method was validated according to the Directive 2002/657/EC and subsequently applied to 56 samples of raw cow milk supplied by the Laboratori Interprofessional Lleter de Catalunya (ALLIC) (Laboratori Interprofessional Lleter de Catalunya, Control Laboratory Interprofessional of Milk of Catalunya). (C) 2011 Elsevier B.V. All rights reserved.
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