Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 879, Issue 25, Pages 2533-2541Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2011.07.006
Keywords
High-performance liquid chromatography-mass spectrometry; Juvenile hormone III; 20-Hydroxyecdysone; Queen larvae; Drone pupae
Funding
- Apicultural Industry Technology System Construction of Modern Agriculture, China [NYCYTX-43]
- Ministry of Agriculture, China [2011-G5-4]
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In this paper, a method for the rapid and sensitive analysis of juvenile hormone III (JH III) and 20-hydroxyecdysone (20E) in queen larvae and drone pupae samples was presented. Ultrasound-assisted extraction provided a significant shortening of the leaching time for the extraction of JH III and 20E and satisfactory sensitivity as compared to the conventional shake extraction procedure. After extraction, determination was carried out by liquid chromatography-tandem mass spectrometry (LC-MS/MS) operating in electrospray ionization positive ion mode via multiple reaction monitoring (MRM) without any clean-up step prior to analysis. A linear gradient consisting of (A) water containing 0.1% formic acid and (B) acetonitrile containing 0.1% formic acid, and a ZORBAX SB-Aq column (100 mm x 2.1 mm, 3.5 mu m) were employed to obtain the best resolution of the target analytes. The method was validated for linearity, limit of quantification, recovery, matrix effects, precision and stability. Drone pupae samples were found to contain 20E at concentrations of 18.0 +/- 0.1 ng/g (mean +/- SD) and JH III was detected at concentrations of 0.20 +/- 0.06 ng/g (mean +/- SD) in queen larvae samples. This validated method provided some practical information for the actual content of JH III and 20E in queen larvae and drone pupae samples. (C) 2011 Elsevier B.V. All rights reserved.
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