Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 879, Issue 3-4, Pages 296-298Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2010.12.015
Keywords
Ion chromatography; Hippuric acid; Urine
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A simple, rapid, precise and eco-friendly ion chromatography (IC) method for the determination of hippuric acid (HA) in human urine was proposed in this paper. The separation was carried out an anion exchange column with 2.0 mmol L-1 Na2CO3 + 2.0 mmol L-1 NaHCO3 as mobile phase at the flow-rate 0.7 mL min(-1). A suppressed conductivity detector was used and the detection limit was 1.0 mu g L-1(S/N = 3) for hippuric acid. The analysis time for one run was 30 min under the optimized IC condition. The recovery of hippuric acid was 93.2-98.0% while the relative standard deviation (RSD) was 1.4-2.3% by seven measurements. (C) 2010 Elsevier B.V. All rights reserved.
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