Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 879, Issue 17-18, Pages 1241-1269Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2011.01.027
Keywords
Amino acids; o-Phthalaldehyde; SH-group containing additives; 9-Fluorenylmethyloxycarbonyl chloride; Derivatization optimizations; High-performance liquid chromatography; HPLC-UV(PDA)/fluorescence/MS detections; Biological tissues
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An overview is presented on the advancement of the two most frequently used derivatization protocols applying the o-phthalaldehyde (OPA)-thiol and the fluorenylmethyloxycarbonyl (FMOC) chloride reagents, prior to the high performance liquid chromatographic analysis of amino acids. This review pays special attention (i) to the blank value, to the composition, to the stability and to the life time of the reagents, (ii) to the optimum pH conditions for the interactions and derivatives' elutions, (iii) to the characteristics and behavior of those amino acids which might provide more than one derivative correlating with the molar ratios of the reagent to the reactants, and (iv) on the practical applicability of the optimized protocols. (C) 2011 Published by Elsevier B.V.
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