Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 879, Issue 20, Pages 1802-1808Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2011.05.002
Keywords
6-Oxy-(acetyl piperazine) fluorescein (APF); Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF); Derivatization; Phytohormones
Funding
- National Natural Science Foundation of China (Beijing, China) [20835004, 30770550]
- Special Research Fund for the Doctoral Program of Higher Education of China [20070486031]
- Fundamental Research Funds for the Central Universities [1101004]
Ask authors/readers for more resources
An efficient and sensitive capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method has been developed for the simultaneous determination of phytohormones containing carboxyl group, including gibberellic acid, indole-3-acetic acid, abscisic acid, jasmonic acid, indole butyric acid, 1-naphthalene acetic acid and 2,4-dichloro-phenoxy acetic acid, based on the chemical derivatization with 6-oxy-(acetypiperazine) fluorescein. Using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide as the condensing reagent, the derivatization reaction completed at 60 degrees C in 60 min and the derivatization limits could reach 20 nmol L(-1). The formed derivatives of seven phytohormones have been separated and quantified within 20 min. The linearity was found in the range of 0.01-1 mu mol L(-1) and the limits of detection were 1.6-6.7 nmol L(-1) (S/N = 3). The proposed method has been applied to analyze the crude extract of 0.5 g banana samples directly without further purification and the recoveries varying from 90.7 to 106.1%. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available