Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 878, Issue 32, Pages 3427-3431Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2010.10.017
Keywords
Curcumin; LC-MS/MS; Liquid liquid extraction (LLE); Solid lipid nanoparticles (SLNs); Plasma
Funding
- Council of Scientific and Industrial Research (CSIR), New Delhi, India [110012]
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A simple and sensitive validated LC-MS/MS analytical method was used for determination of curcumin in rat plasma, using nimesulide as internal standard. Analyses were performed on an Agilent LC-MS/MS system using a Chromolith rod(Tm) and isocratic elution with acetonitrile:10 mM ammonium acetate buffer (pH 3.5) (80:20, v/v) at a flow rate of 0.8 ml/min with a total run time of 3 min and an overall recovery of 77.15%. A triple quadrupole mass spectrometer, equipped with an electrospray ionization interface, operated in the negative mode was used. Calibration curve in plasma spiked with varying concentration of curcumin were linear over the concentration range of 10-2000 ng/ml with determination coefficient >0.99. The lower limit of quantification was 10 ng/ml. Intra and inter-day variability's (RSD) for extraction of curcumin from plasma were less than 10% and 15% respectively and accuracy was 102.43-108.5%. Multiple reaction monitoring was used to monitor the transition for curcumin (m/z: 367/217 [M-H](-)) and IS (m/z: 307/229). The method was applied for determining curcumin concentration in plasma after peroral administration of 50 mg/kg of free curcumin (C-S) or curcumin loaded solid lipid nanoparticles (C-SLNs) to rats. Results established selectivity and suitability of the method for pharmacokinetic studies of curcumin from C-SLNs. (c) 2010 Elsevier B.V. All rights reserved.
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