Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 878, Issue 19, Pages 1531-1536Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2010.04.004
Keywords
Methimazole; Molecularly imprinted polymer; Solid-phase extraction; High performance liquid chromatography
Funding
- Chinese National 863 High-Tech Research Program [2006AA10Z448]
- Natural Science Foundation of Tianjin [09JCYBC14300]
- National Natural Science Foundation of China [20775054, 30872126]
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A novel molecularly imprinted polymer that could be applied as enrichment sorbent was prepared using methimazole (MMZ) as the template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker Though evaluated by static, kinetic and competitive adsorption tests, the polymer exhibited high adsorption capacity, fast kinetics and good selective ability A met hod for determination of trace MMZ was developed using this polymer as enrichment sorbent coupled with high performance liquid chromatography focusing on complex biological matrices Under the optimum experimental conditions, the MMZ standard is linear within the concentration range studied, that is, from 0 5 mu g L-1 to 150 mu g L-1 (r(2) = 0 9941) Lower limits of detection (LOD, at S/N = 3) and quantification (LOQ at S/N = 10) in pig samples were 063 mu g kg(-1) and 2.10 mu g kg(-1) for kidney, 0 51 mu g kg(-1) and 1 70 mu g kg(-1) for liver, 056 mu g kg(-1) and 1 86 mu g kg(-1) for muscle, respectively Recoveries and relative standard deviation (RSD. 11=9) values for precision in the developed method were from 71 14% to 88 41% and from 2 53% to 6 18%. Crown Copyright (C) 2010 Published by Elsevier B V. All rights reserved
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