Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 878, Issue 13-14, Pages 942-948Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2010.02.020
Keywords
Gas chromatograph-mass spectrometer; Organic acidurias; Sample preparation; Solvent extraction; Trimethylsilylation; Flash-heater derivatization
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GC/MS is widely used for the analysis of urinary organic acids for the chemical diagnosis of organic acidurias such as methylmalonic acidemia, propionic acidemia, isovaleric acidemia, glutaric aciduria type I. and multiple carboxylase deficiency. In this study, a rapid and simple preparation method for this analysis was developed in order to improve the laboratory productivity and the working environment. The solvent extraction and trimethylsilylderivatization steps of the conventional method were improved by reducing the volume of urine sample and extraction solvent and by applying the flash-heater derivatization, respectively. The new method was successfully applied to the chemical diagnoses of five organic acidurias. (C) 2010 Elsevier B.V. All rights reserved.
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