Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 878, Issue 7-8, Pages 689-694Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2010.01.007
Keywords
Venlafaxine; O-desmethylvenlafaxine; UPLC-MS/MS; Human plasma; Pharmacokinetics
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A rapid, selective and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to simultaneously determine venlafaxine (VEN) and O-desmethylvenlafaxine (ODV) in human plasma. Sample pretreatment involved a one-step extraction with diethyl ether of 0.5 mL plasma. The separation was carried out on an ACQUITY UPLC (TM) BEH C-18 column with 10 mmol/L. ammonium acetate and methanol as the mobile phase at a flow rate of 0.30 mL/min. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI)source. The linear calibration curves for VEN and ODV were both obtained in the concentration range of 0.200-200 ng/mL (r(2) >= 0.99) with the lower limit of quantification (LLOQ) of 0.200 ng/mL The intra- and inter-day precision (relative standard deviation, R.S.D.) values were less than 13% and the accuracy (relative error, R.E.) was within +/- 5.3% and +/- 3.6% for VEN and ODV. The method herein described was superior to previous methods in sensitivity and sample throughput and successfully applied to clinical pharmacokinetic study of venlafaxine sustained-release capsule in healthy male volunteers after oral administration. (C) 2010 Elsevier B.V. All rights reserved.
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