Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 878, Issue 15-16, Pages 1178-1180Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2010.03.002
Keywords
Selenium; Selenomethionine; NutraSelen (R); HPLC-ICP MS
Funding
- Conseil Regional d'Aquitaine [20071303002PFM]
- FEDER [31486/08011464]
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An analytical method was developed for the simultaneous speciation of selenomethionine (SeMet) and 2-hydroxy-4-methylselenobutanoic acid (NutraSelen (R)), a new SeMet precursor. The compounds could be baseline resolved by ion-pairing reversed-phase HPLC using ICP MS detection. Detection limits of 1 ng mL(-1) (Se content) could be reached. SELM-1 reference material was used to validate the SeMet measurement. Additionally, the quantification of NutraSelen (R) was validated by standard addition together with checking the Se mass balance. The procedure developed was then applied to the monitoring of the conversion of NutraSelen (R) into SeMet by yeast. (C) 2010 Elsevier B.V. All rights reserved.
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