Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 32, Pages 4097-4106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.10.025
Keywords
Microelution SPE plate; Olive oil phenols; Plasma; UPLC-tandem MS
Funding
- Spanish Ministry of Education and Science [AGL2005-07881-C02-01/ALI, AGL2005-07881-C02-02/ALI]
- [BES-2006-14136]
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Two methods based on solid-phase extraction (SPE) using traditional cartridges and microelution SPE plates (mu SPE) as the sample pre-treatment, and an improved liquid chromatography Coupled to tandem mass spectrometry (UPLC-MS/MS) were developed and compared to determine the phenolic compounds in virgin oil olive from plasma samples. The phenolic compounds studied were hydroxytyrosol, tyrosol, homovanillic acid, p-coumaric acid, 3,4-DHPEA-EDA, p-HPEA-EDA. luteolin, apigenin, pinoresinol and acetoxypinoresinol. Good recoveries were obtained in both methods, and the LOQs and LODs were similar, in the range of low mu M. The advantage of mu SPE, in comparison with SPE cartridges, was the lack of the evaporation step to pre-concentrate the analytes. The mu SPE-UPLC-ESI-MS/MS method developed was then applied to determine the phenolic compounds and their metabolites, in glucuronide, sulphate and methylated forms, in human plasma after the ingestion of virgin olive oil. (C) 2009 Elsevier B.V. All rights reserved.
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