Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 4, Pages 456-460Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2008.12.050
Keywords
Disperser liquid-liquid microextraction; Cholesterol; Milk; Yolk; Olive oil; H PLC
Funding
- Research Council of Ilam University [1100]
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A fast, simple, and sensitive sample preparation procedure based on dispersive liquid-liquid microextraction (DLLME) is proposed for the determination of cholesterol in food samples using isocratic reverse phase high performance liquid chromatography (RP-HPLC) and UV detection. The influence of several important parameters on extraction efficiency of cholesterol was evaluated. Under optimized conditions, a linear relationship was obtained between the peak area and the concentration of cholesterol in the range of 0.03-10 mu g l(-1). The detection and quantification limits were 0.01 and 0.03 mu g l(-1), respectively. Intra-day and inter-day precisions for the analysis of cholesterol were in the range of 1.0-3.1%. The applicability of the proposed method was demonstrated by analyzing cholesterol in milk, egg yolk and olive oil. (C) 2008 Elsevier B.V. All rights reserved.
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