Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 8-9, Pages 738-742Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.02.012
Keywords
Ionic liquid-modified silica; Tanshinones; Solid-phase extraction; High-performance liquid chromatography; Salvia Miltiorrhiza Bunge
Funding
- Center for Advanced Bioseparation Technology of Inha University
- Inha University Research Grant, Korea [INHA-2009]
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New ionic liquid-modified silica sorbents were developed by the surface chemical modification of the commercial silica using synthesized ionic liquids. The obtained ionic liquid-modified particles were successfully used as a special sorbent in solid-phase extraction process to isolation of cryptotanshinone. tanshinone I and tanshinone IIA from Salvia Miltiorrhiza Bunge. Different washing and elution solvents such as water, methanol and methanol-acetic acid (90/10, v/v) were evaluated. A comparison of ionic liquid-modified silica cartridges and traditional silica cartridge show that higher recovery was observed using ionic liquid-modified silica sorbents. A quantitative analysis was conducted by high-performance liquid chromatography using a C-18 column (5 mu m. 150 mm x 4.6 mm) with methanol-water (78:22, v/v, and containing 0.5% acetic acid) as a mobile phase. Good linearity was obtained from 0.5 x 10(-4) to 0.5 mg/mL (r(2) > 0.999) with the relative standard deviations less than 4.8%. (C) 2009 Elsevier B.V. All rights reserved.
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