Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 24, Pages 2493-2498Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.06.022
Keywords
Posaconazole; Bioavailability; Serum; HPLC; Column-switching; On-line sample preparation
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Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (lambda(ex): 261 nm, lambda(em): 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration Curve was obtained over the concentration range of 0.1-5 mg/l using a 50 mu l sample (r(2) = 0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%. (C) 2009 Elsevier B.V. All rights reserved.
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