Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 24, Pages 2571-2578Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.06.042
Keywords
prep-HSCCC/ESI-MSn; Coumarins; Peucedanum praeruptorum Dunn
Funding
- Cultivation Fund of the Key Scientific and Technical Innovation Project
- Ministry of Education of China [707033]
- National Key Scientific and Technological Special Projects [2009ZX09502-011]
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A new and systematic application for separation and online identification of coumarins from Peucedanum praeruptorum Dunn by preparative high-speed counter-current chromatography coupled with electrospray ionization multi-stage mass spectrometry (prep-HSCCC/ESI-MSn) was established. The procedure of separation was guided by the chromatogram of ion current. The structures of acquisitions were deduced by MS information. The hyphenation between prep-HSCCC/ESI-MSn was designed to keep the split ratio from 1:20 to 1:200 exactly. Seven compounds were obtained and two new compounds were detected. It was proved that prep-HSCCC/ESI-MSn was an effective method for sensitive detection, rapid identification and separation of natural products. (C) 2009 Elsevier B.V. All rights reserved.
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