Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 1-2, Pages 101-104Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2008.11.027
Keywords
Arabidopsis; Disulfide bonds; Thiol labeling; Proteomics; Mass spectrometry
Funding
- University of Florida
- National Science Foundation [MCB 76708]
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Protein thiol-disulfide oxidoreduction plays an important role in redox regulation of cellular processes. Here we present a proteomic approach to visualize and map in vivo disulfide-bonded proteins in plants. A proteomic map of the disulfide-bonded proteins was achieved using 2D gel electrophoresis of Arabidopsis protein extract. Along with novel proteins identified as potentially redox regulated, we have also shown the feasibility of mapping some of the cysteines involved in the formation of disulfide bonds. This study presents an important tool for characterizing redox-regulated proteins. (C) 2008 Elsevier B.V. All rights reserved.
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