Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 5-6, Pages 482-488Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.12.053
Keywords
Coenzyme A; Acyl-CoA; LC-MS-MS; Plants; Metabolites
Funding
- Metabolix Inc.
- National Science Foundation [MCB0416730]
- Hatch Act and State of Iowa
- Div Of Engineering Education and Centers
- Directorate For Engineering [813570] Funding Source: National Science Foundation
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Because acyl-CoAs play major roles in numerous anabolic and catabolic pathways, the quantitative determination of these metabolites in biological tissues is paramount to understanding the regulation of these metabolic processes. Here, we report a method for the analysis of a collection of short-chain acyl-CoAs (<6 carbon chain length) from plant extracts. Identification of each individual acyl-CoA was conducted by monitoring specific mass-fragmentation ions that are derived from common chemical moieties of all Coenzyme A (CoA) derivatives, namely the adenosine triphosphate nucleotid, pantothenate and acylated cysteamine. This method is robust and quick, enabling the quantitative analysis of up to 12 different acyl-CoAs in plant metabolite extracts with minimal post-extraction processing, using a 30 min chromatographic run-time. (C) 2008 Elsevier B.V. All rights reserved.
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