Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 27, Pages 3065-3071Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.07.028
Keywords
Methadone; Cocaine; Opiates; Umbilical cord; LCMS; In utero
Funding
- National Institutes of Health
- Intramural Research Program of the National Institute on Drug Abuse
- Direccion Xeral de Investigacion, Desenvolvemento a Innovacion (Conselleria de Innovacion a Industria), Galicia, Spain
- NATIONAL INSTITUTE ON DRUG ABUSE [ZIADA000433, ZIADA000412] Funding Source: NIH RePORTER
Ask authors/readers for more resources
A liquid chromatography mass spectrometric selected reaction monitoring mode (SRM) method for methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), cocaine, benzoylecgonine (BE), 6-acetylmorphine, morphine and codeine quantification in human umbilical cord was developed and fully validated. Analytes were extracted from homogenized tissue (1 g) by solid phase extraction. Linearity was 2.5-500 ng/g, except for methadone (10-2000 ng/g). Method imprecision was <12.7%CV with analytical recovery 85.9-112.7%, extraction efficiency >59.2%, matrix effect 4.5-39.5%, process efficiency 48.6-92.6% and stability >84.6%. Analysis of an umbilical cord following controlled methadone administration and illicit drug use contained in ng/g, 40.3 morphine, 3.6 codeine, 442 BE, 186 methadone and 45.9 EDDP. Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available