Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 1-2, Pages 71-78Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.11.030
Keywords
Prenylflavonoids; Liquid chromatography-mass spectrometry (LC-MS); Rat serum; Epimedium
Funding
- Dr. Willmar Schwabe Pharmaceuticals (Karlsruhe, Germany)
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A rapid and sensitive method to separate and quantify icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera was developed using liquid chromatography-tandem mass spectrometry. Serum samples were extracted with ethyl acetate without further derivatization. Using coumestrol as an internal standard, calibration curves with good linearity (r(2) > 0.99) within the concentration range of 0.78-12.5 nM for icariin, icaritin and desmethylicaritin, and 0.78-100 nM for icariside I and 11, were obtained in the multiple reaction monitoring mode. For all analytes, the limits of detection and quantification were < 1 nM and 1-2 nM, respectively. Inter- and intra-assay variabilities were < 15% and accuracies were between 94% and 114%, respectively. This method was successfully applied to quantify levels of icadin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera after oral administration of an Epimedium preparation. (C) 2008 Elsevier B.V. All rights reserved.
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