Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 875, Issue 2, Pages 546-550Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.09.019
Keywords
Daptomycin; HPLC; UV; Stability
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Daptomycin is the first approved member of the new class cyclic lipopeptide antibiotic drugs, effective against a broad spectrum of Gram-positive bacteria. Here we present an HPLC method with UV detection capable to obtain pharmacokinetic data of daptomycin in human plasma, exemplarily shown in a critically ill patient with acute renal failure undergoing extended daily dialysis. Sample preparation consists only of protein precipitation with methanol. Chromatographic separation was achieved on a Zorbax Eclipse XDB-C8 column and daptomycin was detected at 224 nm. The calibration function was linear over the range from 3.5 to 350 mu g/ml. The relative standard deviations were < 2% in the intra-day and < 6% in the inter-day measurements. The accuracy was always better than 7%. Daptomycin was stable in aqueous solutions for 2 months frozen at -20 degrees C. However, in plasma frozen at -20 degrees C a loss of 25% in 1 month was observed. (c) 2008 Elsevier B.V. All rights reserved.
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