Identification of volatile organic compounds secreted from cancer tissues and bacterial cultures

The early cancer diagnosis increases the possibility of total recovery. The infection of Helicobacter pylori is associated with gastric cancer, the second most common cancer in the world. The determination of volatile organic compounds (VOCs) excreted by stomach tissue and bacteria culture has been investigated. Solid-phase microextraction (SPME) was used for preconcentration and the determination was accomplished by gas chromatography coupled with mass spectrometry (GC/MS). The samples of tissue were taken from five patients (ten samples) with stomach cancer and normal (non-cancerous) segments from other parts of the stomach were used as a control. Eighteen compounds were identified in stomach tissue and seven of them were present both in healthy and cancer tissue. These compounds assumed to be enclogenous and acetone ratio (AR) was calculated for ethanol, butane, carbon disulficle, 1-propanol, 2-butanone and 2pentanone.The data shows thatamountof 1-propanol and carbon disulfide inthegasecrus composition is higher in cancer tissue than in normal tissue. Eight compounds were identified both in bacteria and tissue. These data suggest that bacteria present in the stomach might cause the increase in the concentration of I -propanol and carbon disulfide in emission from cancer tissue. (c) 2008 Elsevier B.V. All rights reserved.