Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 867, Issue 1, Pages 105-110Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.03.025
Keywords
besifloxacin; fluoroquinolone; antimicrobial agent; human tears; LC/MS/MS; pharmacokinetics
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A rapid and sensitive method was developed using high-performance liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) for the quantification of besifloxacin in human tears using sparfloxacin as the internal standard (IS). Besifloxacin was extracted from human tear samples using an ammonium formate buffer at pH 3.25. The method was validated over a concentration range of 2-2000 ng/mL, with a total run time of less than 4 min. The overall intra- and inter-day precision for this method was less than 6%. The method was used to measure besifloxacin concentrations in tear samples collected after topical ocular administration to humans; besifloxacin concentrations were 610 +/- 540 mu g/g (15 min) and 1.60 +/- 2.28 mu g/g (24 h). (C) 2008 Elsevier B.V. All rights reserved.
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