Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 862, Issue 1-2, Pages 205-212Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2007.12.009
Keywords
colistin; LC-MS/MS; SPE; plasma; urine
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A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to quantify colistin in human plasma and urine, and perfusate and urine from the isolated perfused rat kidney (IPK). Solid phase extraction (SPE) preceded chromatography on a Synergi Fusion-RP column with a mobile phase of acetonitrile, water and acetic acid (80/19/1) at 0.2 mL/min. Ions were generated using electrospray ionization and detected in the positive-ion mode. Multiple reaction monitoring was performed using precursor-product ion combinations. Calibration curves were linear from 0.028 mu g/mL (human plasma, IPK perfusate and urine)/0.056 mu g/mL (human urine) to 1.78 mu g/mL (all four media) for colistin A sulfate; corresponding values for colistin B sulfate were 0.016/0.032 to 1.01 mu g/mL. Accuracy and precision were within 10%. The LLOQ for colistin A sulfate was 0.028 mu g/mL in human plasma, IPK perfusate and urine and 0.056 mu g/mL in human urine; corresponding values for colistin B sulfate were 0.016 and 0.032 mu g/mL. The low sample volume, short analysis time and low LLOQ are ideal for pre-clinical and human pharmacokinetic studies of colistin. Crown Copyright (c) 2007 Published by Elsevier B.V. All rights reserved.
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