Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 869, Issue 1-2, Pages 67-74Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.05.021
Keywords
iodine-azide reaction; methimazole assay; urine; HPLC
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The iodine-azide detection system to determine methimazole following its separation by RP-HPLC is described in this paper. The reaction between iodine and azide ions induced by methimazole was applied as a post-column reaction detection system. Neither extraction nor preconcentration of the sample was necessary. The methimazole standards added to normal urine show that the response of the detector, set at 350 nm (corresponding to unreacted iodine in the post-column iodine-azide reaction), was linear within the concentration range 2-10 nmol/mL of urine. The relative standard deviation values for precision and recovery within the calibration range were from 0.3 to 3.2% and from 97 to 102%, respectively. Limits of detection (LOD) and quantitation (LOQ) were 1 and 2 nmol/mL of urine, respectively. The method was applied to the separation and determination of patient urine samples and the analytical results were satisfactory. (c) 2008 Elsevier B.V. All rights reserved.
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