Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 862, Issue 1-2, Pages 86-92Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2007.11.003
Keywords
spent cell culture medial; amino acids derivatives; dipeptide derivatives; EZ : faast kit; liquid phase extraction; solid phase extraction; gas chromatography-flame ionization detector
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A rapid, sensitive and reproducible gas chromatographic method with flame ionization detection is described for the simultaneous identification and quantification of 33 amino acids and dipeptides in spent cell culture media in under seven minutes. The method involves the use of the EZ:faast (TM) (Phenomenex) amino acid sample testing kit. Instrumental and assay precision, percent recovery, linear range, limit of detection and peak identity in highly complex cell culture media containing either soy hydrolysate or fetal bovine serum were validated using gas chromatography-flame ionization detector (GC-FID). (c) 2007 Elsevier B.V. All rights reserved.
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