Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1331, Issue -, Pages 90-99Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.01.044
Keywords
Carotenoid profile; UPLC; Monovarietal fruit juice; Epoxycarotenoid; Hydroxycarotenoid
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We have developed an analytical method that allows the simultaneous determination of epoxy-carotenoids, hydroxycarotenoids and carotenes in monovarietal fresh homemade and industrially processed fruit products. Analyses were carried out using ultra performance liquid chromatography (UPLC). The extraction method was optimized using methanol as the first extraction solvent for lyophilized samples followed by a saponification step. Recoveries ranged between 75% and 104% depending on the compound. Repeatability was better than 10% for all compounds (%RSD, n=3). The chromatographic analysis takes less than 17 min. In this short period, up to 27 carotenoids were identified in apple, peach and pear products. The developed method allowed us to differentiate juice from six varieties of apple by their carotenoid profile. Moreover, the methodology allows us to differentiate the carotenoid profiles from commercial juices and homemade fresh peach and pear juices, as well as to study the rearrangements of 5,6- to 5,8-epoxycarotenoids. (C) 2014 Elsevier B.V. All rights reserved.
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