Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1374, Issue -, Pages 1-13Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.11.050
Keywords
Metabolomics; Metabolic profiling; LC-MS; Metabolite coverage; Pseudo-targeted method
Funding
- National Basic Research Program of China [2012CB518303]
- State Key Science & Technology Projects for Infectious Diseases [2012ZX10002-011, 2012ZX10002-009]
- National Natural Science Foundation of China [21375127, 21205114, 21321064]
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Metabolomics, as a part of systems biology, has been widely applied in different fields of life science by studying the endogenous metabolites. The development and applications of liquid chromatography (LC) coupled with high resolution mass spectrometry (MS) greatly improve the achievable data quality in non-targeted metabolic profiling. However, there are still some emerging challenges to be covered in LC-MS based metabolomics. Here, recent approaches about sample collection and preparation, instrumental analysis, and data handling of LC-MS based metabolomics are summarized, especially in the analysis of clinical samples. Emphasis is put on the improvement of analytical techniques including the combination of different LC columns, isotope coded derivatization methods, pseudo-targeted LC-MS method, new data analysis algorithms and structural identification of important metabolites. (C) 2014 Elsevier B.V. All rights reserved.
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