Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1373, Issue -, Pages 90-96Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.11.015
Keywords
HILIC separation; ESI-MS; Monolithic silica column; Nucleobase; Nucleoside; Nucleotide
Funding
- Ministry of the Interior of the Czech Republic [VG20112015021]
- Czech Science Foundation [P106/12/0522]
- Academy of Sciences of the Czech Republic [RVO: 68081715]
- Magnus Ehrnrooth Foundation
- Academy of Finland [266342, 257316]
- Academy of Finland (AKA) [257316] Funding Source: Academy of Finland (AKA)
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The capability of employing synthesized zwitterionic silica-based monolithic capillary columns (140 mm x 0.1 mm) for separation of highly polar and hydrophilic nucleobases, nucleosides, and nucleotides in hydrophilic interaction chromatography is reported. The suitability of the columns for on-line conjunction with electrospray tandem mass spectrometry was explored. Our results show that the grafted layer of zwitterionic monomer ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide or 2-methaciyloyloxyethyl phosphorylcholine) on the silica monolithic surface significantly improved the separation selectivity and reproducibility, as compared to the bare silica monolith. The stepwise elution from 90% to 70% of acetonitrile enabled separation of a complex sample mixture containing 21 compounds with a total analysis time less than 40 min. (C) 2014 Elsevier B.V. All rights reserved.
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