Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1326, Issue -, Pages 1-6Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2013.12.037
Keywords
Micro extraction; Spermidine; Spermine; Urine; Blood
Funding
- National Science Council [NSC 102-2113-M-037-010]
- NSYSU-KMU JOINT RESEARCH PROJECT [NSYSUKMU102-P010]
- Center of Excellence for Environmental Medicine (Kaohsiung Medical University)
Ask authors/readers for more resources
Spermidine and its derivative, spermine, are basic compounds with unique roles in physiological function. This study used matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to monitor these analytes in human urine and blood. The MALDI-TOF MS is also suitable when a high-throughput analytical method is required. Unlike liquid chromatography (LC)-MS, MALDI-TOF MS does not require a mobile phase in sample separation and generates very little organic waste. Micro liquid-liquid extraction was also performed to minimize the use of organic solvents in this study. After alkalization step, biosamples were prepared by adding a small volume (20 mu L) of organic solvent to concentrate, and purify the spermidine and spermine contained in the urine and blood samples. A suitable extraction protocol was developed after optimizing various conditions associated with extraction efficiency. The proposed method was then successfully used to monitor these compounds in human urine (mean value < 1 mu g/mL) and blood (mean value > 1 mu g/mL). (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available