Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1357, Issue -, Pages 182-193Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2014.04.078
Keywords
Core-shell structure; Magnetic microspheres; Enrichment; Low-abundance proteins/peptides; Phosphoproteins; Glycoproteins
Funding
- National Basic Research Priorities Program [2012CB910602, 2013CB911201]
- National Natural Science Foundation of China [21075022, 21275033, 21105016]
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 20100071120053]
- National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China [J1103304]
- Graduate Innovation Fund of Fudan University
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Many endogenous proteins/peptides and proteins/peptides with post-translational modifications (PTMs) are presented at extremely low abundance, and they usually suffer strong interference with highly abundant proteins/peptides as well as other contaminants, resulting in low ionization efficiency in MS analysis. Therefore, the separation and enrichment of proteins/peptides from complex mixtures is of great importance to the successful identification of them. Core-shell structured magnetic microspheres have been widely used in the enrichment and isolation of proteins/peptides, thanks to unique properties such as strong magnetic responsiveness, outstanding binding capacity, excellent biocompatibility, robust mechanical strength and admirable recoverability. The aim of this review is to update the advances in the application of core-shell structured magnetic materials for proteomics analysis, including the separation and enrichment of low-concentration proteins/peptides, the selective enrichment of phosphoproteins and the selective enrichment of glycoproteins, and to compare the enrichment performance of magnetic microspheres with different kinds of functionalization. (C) 2014 Elsevier B.V. All rights reserved.
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