Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1324, Issue -, Pages 128-134Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2013.11.029
Keywords
High performance liquid chromatography; Monolithic column; N-isopropylacrylamide; Small molecule compounds
Funding
- Natural Science Foundation of Hebei University [B2013201082]
- Key Project Foundation of Hebei Province Higher Education [ZD2010234]
- Key Basic Research Foundation of Hebei Province [11966411D]
- National Natural Science Foundation of China [21175031, 2013-247]
- Pharmaceutical Joint Research Foundation of the Natural Science Foundation of Hebei Province and China Shiyao Pharmaceutical Group Co., Ltd. [B2011201174]
- issue of second batch of twelfth Five-year Plan of Key Special Project for significant Drug Discovery of Ministry of Science and Technology of China [2012ZX09103-101-057]
- funds of Education Department of Hebei province [Z2013112]
- Natural Science Foundation of Hebei Province [B2012201052]
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A porous monolith was prepared by in situ free-radical polymerization using N-isopropylacrylamide (NIPAAm) and trimethylol propane triacrylate (TMPTA) as functional monomers, ethylene dimethacrylate (EDMA) as crosslinking agent. The chemical group of the monolith was assayed by a Fourier transform infrared spectroscopy (FT-IR) method and the morphology of optimized monolithic column was characterized by scanning electron microscopy (SEM). The mechanical strength and permeability have been studied in detail as well. The run-to-run and column-to-column reproducibility of the retention times were less than 0.9% and 3.0%, respectively. Furthermore, the influence of temperature and mobile phase composition on the separation of aromatic compounds was investigated. The results indicated that poly (trimethylol propane triacrylate-co-N-isopropylacrylamide-co-ethylenedimethacrylate) (TMPTA-co-NIPAAm-co-EDMA) monolithic column not only had high porosity and strong rigidity, but also was a promising tool for analyzing small molecule compounds with a short analysis time by controlling the column temperature. (C) 2013 Elsevier B.V. All rights reserved.
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