Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1304, Issue -, Pages 211-219Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.06.051
Keywords
Asymmetrical flow field-flow fractionation (AF4); Steric transition; Normal mode; Steric/hyperlayer mode; RDX particles
Funding
- Hannam University
- Korea Research Foundation (KRF)
- research project for Environmental Risk Assessment of Manufactured Nanomaterials [KK-1303-03]
- Korea Institute of Toxicology (KIT, Korea)
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In field-flow fractionation (FFF), there is the 'steric transition' phenomenon where the sample elution mode changes from the normal to steric/hyperlayer mode. Accurate analysis by FFF requires understanding of the steric transition phenomenon, particularly when the sample has a broad size distribution, for which the effect by combination of different modes may become complicated to interpret. In this study, the steric transition phenomenon in asymmetrical flow FFF (AF4) was studied using polystyrene (PS) latex beads. The retention ratio (R) gradually decreases as the particle size increases (normal mode) and reaches a minimum (R-i) at diameter around 0.5 mu m, after which R increases with increasing diameter (steric/hyperlayer mode). It was found that the size-based selectivity (S-d) tends to increase as the channel thickness (w) increases. The retention behavior of cyclo-1,3,5-trimethylene-2,4,6-trinitramine (commonly called 'research department explosive' (RDX)) particles in AF4 was investigated by varying experimental parameters including wand flow rates. AF4 showed a good reproducibility in size determination of RDX particles with the relative standard deviation of 4.1%. The reliability of separation obtained by AF4 was evaluated by transmission electron microscopy (TEM). (C) 2013 Elsevier B.V. All rights reserved.
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