Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1282, Issue -, Pages 161-166Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.01.066
Keywords
Capillary electrophoresis; Chemiluminescence detection; Gold nanoparticles; Immunoassay; Carcinoembryonic antigen
Funding
- National Natural Science Foundations of China [21175030]
- Natural Science Foundations of Guangxi Province [2010GXNSFF013001]
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A noncompetitive immunoassay based on gold nanoparticles (AuNPs) amplified capillary electrophoresis (CE) chemiluminescence (CL) detection was developed for the determination of carcinoembryonic antigen (CEA). In this method, citrate-modified AuNPs were conjugated with horseradish peroxidase (HRP) labeled CEA antibody (Ab*), and incubated with limited amount of CEA antigen. CEA-Ab*-AuNPs complex and excess of Ab*-AuNPs were then separated and quantified by CE with CL detection. Highly sensitive CL detection was achieved by means of p-iodophenol (PIP) enhanced luminol-H2O2-HPR CL reaction and AuNPs amplified. Under the optimal conditions, the CE assay was accomplished within 5 min. The linear range for CEA detection was 0.05-20 ng/mL with a detection limit of 0.034 ng/mL (signal/noise = 3), which is three orders magnitude lower than that of without AuNPs amplified. The current method was successfully applied for the quantification of CEA in human serum samples. It was demonstrated that the current CE-CL AuNPs amplified noncompetitive immunoassay was sensitive and highly selective. It may serve as a tool for clinical analysis of CEA to assist in the diagnosis of cancer. (C) 2013 Elsevier B.V. All rights reserved.
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