Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1315, Issue -, Pages 61-69Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2013.09.057
Keywords
Endogenous phosphopeptides; Human serum; Metal oxide affinity chromatography; Titanium-grafted magnetic mesoporous silica
Funding
- National Basic Research Program of China (973 Program) [2013CB910702, 2012CB720601]
- National Natural Science Foundation of China [91217309, 91017013, 31070327, 21205091, 21228501]
- Fundamental Research Funds for the Central Universities
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As one of the most important post-translational modifications, reversible phosphorylation of protein plays crucial roles in a large number of biological processes. Moreover, endogenous phosphopeptides are also associated with certain human diseases. An efficient enrichment and separation method is the premise for successful identification and quantification of phosphopeptides. In this work, titanium grafted magnetic mesoporous silica (Fe3O4@Ti-mSiO(2)) was developed and applied for the enrichment of endogenous phosphopeptides. Fe3O4@Ti-mSiO(2) particles were prepared by grafting titanocene dichloride on the inner walls of magnetic mesoporous silica and then being calcined to remove cyclopentadienyl ligand. The physicochemical properties of the prepared materials were characterized by energy dispersive X-ray spectroscopy (EDX), nitrogen adsorption-desorption analysis, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM). For selective enrichment of phosphopeptides, the prepared Fe3O4@Ti-mSiO(2) particles were applied for tryptic digests of beta-casein, mixtures of beta-casein and bovine serum albumin (BSA), and low-fat milk. Finally, Fe3O4@Ti-mSiO(2) was successfully applied for the enrichment of endogenous phosphopeptides from human serum. (C) 2013 Elsevier B.V. All rights reserved.
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