Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1274, Issue -, Pages 44-53Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.12.011
Keywords
Molecularly imprinted polymers; Magnetic carbon nanotubes; Magnetic solid phase extraction; Gatifloxacin; Serum samples
Funding
- Graduate Students Innovative Projects of Jiangsu Province [CXZZ11_0812]
- Guizhou Provincial Natural Science Foundation of China [J20122288]
- Zhejiang Provincial Natural Science Foundation of China [Y4110235]
- Fundamental Research Funds for the Central Universities [JKY2011008]
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A novel composite imprinted material, on the basis of magnetic carbon nanotubes (MCNTs)-incorporated layer using gatifloxacin as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker, was successfully synthesized by a surface imprinting technique. Adsorption dynamics and a Scatchard adsorption model were employed to evaluate the adsorption process. The results showed that magnetic carbon nanotubes molecularly imprinted polymers (MCNTs@MIP) displayed a rapid dynamic adsorption and a high adsorption capacity of 192.7 mu g/mg toward GTFX. Applied MCNTs@MIP as a sorbent, a magnetic solid phase extraction method coupled with high performance liquid chromatography (MSPE-HPLC) was developed for the determination of GTFX in serum samples. The recoveries from 79.1 +/- 4.8% to 85.3 +/- 4.2% were obtained. MCNTs@MIP can not only be collected and separated fast by external magnetic field but also have high surface-to-volume ratio, outstanding mechanical properties and specific recognition toward template molecule. In addition, the MCNTs@MIP could be regenerated, which could be used for five cycles with lost of less than 7.8% of its recovery on average. These analytical results of serum samples display that the proposed method based on MCNTs@MIP is applicable for fast and selective extraction of therapeutic agents from biological fluids. (C) 2012 Elsevier B.V. All rights reserved.
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