Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1310, Issue -, Pages 37-44Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2013.07.102
Keywords
LC x LC; Analysis time; Crossover time; Peak capacity; Cycle time; Fractional coverage
Funding
- National Institutes of Health [5R01GM54585-15]
- National Science Foundation [CHE-0911330]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1213561] Funding Source: National Science Foundation
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Our purpose is to generalize the chief conclusion of earlier studies based on complex maize seed extract samples that indicated that at very short times (less than 5 min) the peak capacity of 1D liquid chromatography was better than that of comprehensive, on-line 2D liquid chromatography (LC x LC), but that the LC x LC method begins to out-perform the 1D method at surprisingly short analysis times of only 5-10 min. We call the analysis time at which the peak capacities of 1D and LC x LC become equal the crossover time. We quantify the importance of various system parameters such as the 10 peak capacity, the second dimension cycle time and second dimension peak capacity, and fractional coverage of the two-dimensional separation space on the crossover time of 10 versus LC x LC chromatography. For ranges of these parameters used in our earlier experimental studies, we believe that crossover times will generally be between 3 and 8 min under optimized on-line LC x LC conditions. (C) 2013 Elsevier B.V. All rights reserved.
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