Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1238, Issue -, Pages 54-59Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2012.03.022
Keywords
LC-ESI/MS; Staphyloccocus aureus; Staphylococcal enterotoxins; Milk; Fruit juice
Funding
- Conselleria de Sanitat-Generalitat Valenciana [072/2009]
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Staphylococcus aureus is an important pathogen and has been indicated as the fifth causative agent of food-borne human illness throughout the world. Staphylococcal enterotoxins (SEs) are toxic compounds excreted mainly by strains of S. aureus. Among these toxins, enterotoxins A (SEA) and B (SEB) are both of the most prevalent compounds in staphylococcal food poisoning. In this work, reverse phase liquid chromatography coupled to ESI mass spectrometry (LC-ESI/MS) has been applied for its rapid identification and quantification. Limit of detection (LOD) values were 0.5 and 0.2 ng for SEA and SEB, respectively and limit of quantification (LOQ) value was 1 ng for both enterotoxins. SEA and SEB have been analyzed as intact proteins in milk and fruit juices. Analytical methods are essential for routine monitoring purposes and safeguard public health and the proposed technique can detect and quantify successfully SEA and SEB in food samples. (C) 2012 Elsevier B.V. All rights reserved.
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