Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1255, Issue -, Pages 237-243Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.11.062
Keywords
Comprehensive two-dimensional liquid chromatography; Hydrophilic interaction liquid chromatography; Ion-pair reversed-phase liquid chromatography; LC x LC interface; Oligonucleotides
Funding
- Key Foundation [20835006]
- National Natural Science Foundation of China [21021004]
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A comprehensive two-dimensional HPLC approach with a high degree of orthogonality was developed for analysis of di- to deca-oligonucleotides (ONs). Hydrophilic interaction liquid chromatography (HILIC) was used in the first dimension, and ion-pair reversed-phase liquid chromatography (IP-RPLC) was employed in the second dimension. The two dimensions were connected via a ten-port valve interface equipped with octadecyl silica (ODS) traps to immobilize and focus the ONs eluting from the first dimension prior to IP-RPLC separation. An aqueous make-up flow was used for effective trapping. The comprehensive two-dimensional HPLC system was optimized with a mixture consisting of 27 oligonucleotide standards. An overall chromatographic peak capacity of 500 was obtained. The use of the volatile buffer triethylamine acetate in the second dimension allowed straightforward coupling to electrospray ionization mass spectrometry (ESI-MS) and detection of each ON in the negative ionization mode. (C) 2011 Elsevier B.V. All rights reserved.
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