Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1256, Issue -, Pages 15-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.07.047
Keywords
Polymer monolith microextraction; High performance liquid chromatography; Manual on-line system; Chlorophenols; 4-Vinylpyridine; Environmental and biological samples
Funding
- National Basic Research Program of China (973 program) [2009CB421601, 2011CB911003]
- National Natural Science Foundation of China [90913012]
- National Science Funds for Creative Research Groups [21121091]
- Nanjing University
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A simple and facile needle-adapter was designed for constructing manual on-line polymer monolith microextraction-high performance liquid chromatography (PMME-HPLC). A capillary poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) [poly(VP-co-EGDMA)] monolith was prepared by in situ polymerization, using 4-vinylpyridine (VP) and ethylene glycol dimethacrylate (EGDMA) as functional monomer and cross-linker, respectively. The synthesized monolith was used as the extraction medium for concentrating four EPA priority pollutants, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol, from water samples. The effect of the dosage of porogen polyethylene glycol 6000 (PEG) on back pressure and extraction performance of the capillary monolith was also investigated. Moreover, the influences of several parameters (such as extraction time, desorption time, content of MeOH in sample solution and sample pH) were examined to obtain the optimal PMME conditions. As a result, the established on-line PMME-HPLC protocol, with good extraction efficiency (80.6-91.7%), satisfactory recovery (94.7-106% and 76.8-86.3% for water and serum samples, respectively) and low detection limit (0.3-1.4 mu g/L). exhibited potential applicability for the analysis of chlorophenols in environmental and biological samples. (C) 2012 Elsevier B.V. All rights reserved.
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