Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1261, Issue -, Pages 78-90Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.06.098
Keywords
Liquid chromatography; Proteomics; Peptides; Proteins; Column technologies
Funding
- National Center for Research Resources [5P41RR018522-10]
- National Institute of General Medical Sciences [8 P41 GM103493-10]
- National Cancer Institute from the National Institutes of Health [U24-CA-160019-01]
- DOE [DE-AC05-76RLO-1830]
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Liquid chromatography coupled with mass spectrometry is the predominant platform used to analyze proteomics samples consisting of large numbers of proteins and their proteolytic products (e.g., truncated polypeptides) and spanning a wide range of relative concentrations. This review provides an overview of advanced capillary liquid chromatography techniques and methodologies that greatly improve separation resolving power and proteomics analysis coverage, sensitivity, and throughput. (C) 2012 Elsevier B.V. All rights reserved.
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