Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1270, Issue -, Pages 334-339Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2012.10.060
Keywords
Patulin; Mycotoxin; Mass spectrometry; ESI; Fragmentation; Adduct formation
Funding
- Special Research Fund of Ghent University (BOF) [01J08409, 01D12611]
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Several sensitive methods have been developed for patulin determination; however, mass spectrometric (MS) detection of this toxin in the positive electrospray ionization (ESI*) mode is not straightforward. Furthermore, the combined determination of patulin with other mycotoxins in one single run has not been reported yet. The present paper demonstrates the formation and use of a methanol adduct of patulin in ESI*. A study of the fragmentation pathway confirmed the authenticity of the patulin adduct, while the use of ion trap and high resolution Orbitrap mass spectrometry allowed reliable assignment of the patulin fragment ions. Exploiting the formation of the methanol adduct, patulin has been successfully included in a single run multi-mycotoxin liquid chromatography tandem mass spectrometric (LC-MS/MS) method in support of ex vivo-in vitro biomedical studies. (C) 2012 Elsevier B.V. All rights reserved.
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