Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1224, Issue -, Pages 11-18Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.12.045
Keywords
Magnetic mesoporous silica; Pseudomorphic transformation; Enrichment of endogenous peptides; MALDI-TOF mass spectrometry
Funding
- National Natural Science Fundation of China [91017013, 31070327]
- Science Fund for Creative Research Groups [20921062]
- NSFC
- Fundamental Research Funds for the Central Universities
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In this work, we describe a novel synthetic strategy of magnetic mesoporous silica spheres (Fe3O4@mSiO(2)) for the selective enrichment of endogenous peptides. Fe3O4 particles were coated with silica shell by a sol-gel method, followed by pseudomorphic synthesis to transform nonporous silica shell into ordered mesoporous silica shell. The core/shell structure and mesostructure were individually fabricated in two steps, which can be expedient to independently optimize the properties of monodispersion, magnetization and mesostructure. Actually, it was confirmed that the produced Fe3O4@mSiO(2) particles possess good monodispersion, high magnetization, superparamagnetism, uniform accessible mesopores, and large surface area and pore volume. With these good properties, Fe3O4@mSiO(2) spheres were applied to the rapid enrichment of peptides. Based on the size-exclusion mechanism and hydrophobic interaction with siloxane bridge group mainly on the surface of inside pores. Fe3O4@mSiO(2) can selectively capture peptides and exclude high-MW proteins and salts. Furthermore, peptides in human plasma were successfully enriched by Fe3O4@mSiO(2). (C) 2011 Elsevier B.V. All rights reserved.
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