Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1256, Issue -, Pages 114-120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2012.07.063
Keywords
Boronate affinity; Cis-diol containing compounds; Enrichment; Monolithic columns; Separation
Funding
- National Natural Science Foundation of China [21075063, 21121091]
- Natural Science Foundation of Jiangsu Province [KB2011054]
- Ministry of Science and Technology of China [34-8]
- Dionex China
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A new boronate-silica hybrid monolithic column was prepared using a one-pot approach with 3-acrylamidophenylboronic acid (AAPBA) as the boronate affinity ligand. The AAPBA-silica monolith exhibited several attractive advantages. First, it is highly hydrophilic, providing excellent specificity and avoiding the presence of organic solvent in the mobile phase. Second, due to its large surface area, it exhibited a high binding capacity, 49.5 mu mol/mL, the highest among the boronate affinity monolithic columns appeared in the literature. Third, the monolith can bind with cis-diol containing compounds at pH as low as 6.5, which not only avoids the use of basic pH conditions at which the silica monolith may hydrolysis but also facilitates the applications to wider sample range. Finally, the hybrid monolithic column exhibited apparent secondary separation capability, which allows for two-dimensional (2D) separation of cis-diol compounds in a single column. Due to these merits, the AAPBA-silica hybrid monolithic column can be a promising separation medium for the analysis of cis-diol containing compounds. (C) 2012 Elsevier B.V. All rights reserved.
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