Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1218, Issue 32, Pages 5527-5530Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2011.04.013
Keywords
Toroidal; TCC; CPC; Counter-current chromatography (CCC); Protein purification; Lysozyme; Myoglobin
Funding
- Biotechnology and Biological Sciences Research Council [BB/FOF/206, BB/C506364/1] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/C506364/1] Funding Source: researchfish
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Two different laboratory scale liquid-liquid extraction processes using aqueous two-phase systems (ATPS) are compared: centrifugal partition chromatography (CPC) and multilayer toroidal coil chromatography (MTCC). Both use the same phase system, 12.5% (w/w) PEG-1000:12.5% (w/w) K2HPO4, the same flow rate of 10 mL/min and a similar mean acceleration field of between 220 x g and 240 x g. The main performance difference between the two processes is that there is a continuous loss of stationary phase with CPC, while for MTCC there is not - even when sample loading is increased. Comparable separation efficiency is demonstrated using a mixture of lysozyme and myoglobin. A throughput of 0.14 g/h is possible with CPC despite having to refill the system with stationary phase before each injection. A higher throughput of 0.67 g/h is demonstrated with MTCC mainly clue to its ability to tolerate serial sample injections which significantly reduces its cycle time. While CPC has already demonstrated that it can be scaled to pilot scale, MTCC has still to achieve this goal. (C) 2011 Elsevier B.V. All rights reserved.
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