Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 52, Pages 8181-8185Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.10.067
Keywords
Monolithic columns; Liquid chromatography; Capillary columns; Size exclusion chromatography; Pore size distribution; Porosity; Biocompatible; Polyethylene glycol diacrylate; Brij; Proteins; Peptides
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Biocompatible poly(ethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monoliths were prepared for size exclusion chromatography (SEC) of proteins in the capillary format using Brij 58P in a mixture of hexanes and dodecanol as porogens. The monolithic columns provided size separation of four proteins in 20 mM sodium phosphate buffer (pH 7.0) containing 0.15 M NaCl, and there was a linear relationship between the retention times and the logarithmic values of the molecular weights. Compared to SEC monoliths previously synthesized using a triblock copolymer of polyethylene oxide and polypropylene oxide, an increase in mesoporosity was confirmed by inverse size exclusion chromatography. As a result, improved protein separation in the high molecular weight range and reduced column back-pressure were observed. (C) 2010 Elsevier B.V. All rights reserved.
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