Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 7, Pages 990-999Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2009.10.021
Keywords
Chiral stationary phase (CSP); Enantioselective HPLC; Enantioselective UHPLC; Fast HPLC; Reduced particle size; Sub-2-micron particles
Funding
- FIRB [RBPR05NWWC_003]
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Three brush-type chiral stationary phases (CSPs) differing in the particle size of the starting silica particles have been prepared by covalent grafting of the pi-acidic bis-(3,5-dinitrobenzoyl)-derivative of trans-1,2-diaminocyclohexane (DACH-DNB). Starting silica particles of 4.3, 2.6 and 1.9 micron were used to generate the final CSPs using an improved, highly reproducible synthetic methodology, that allowed to assemble and surface-graft the whole chiral selector in only two steps. The different CSPs have been packed in columns of various length and diameters, and fully characterized in terms of flow permeability, kinetic performances and enantioselectivity using a set of test solutes. Very high speed and high resolution applications together with stereodynamic HPLC examples are demonstrated on the columns with reduced particle diameters, on which separations of several enantiomeric pairs are routinely obtained with analysis times in the 15-40s range. (c) 2009 Elsevier B.V. All rights reserved.
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