Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 27, Pages 4612-4622Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.05.007
Keywords
Anthelmintics; UHPLC-MS/MS; Polarity switching; QuEChERS; Milk
Funding
- Irish Department of Agriculture, Fisheries and Food [06RDTMFRC434, 06RDTAAFRC479]
- EU [FOOD-CT-2006-36241]
- Federal Office of Consumer Protection and Food Safety (BVL) (European Community Reference Laboratory)
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A new UHPLC-MS/MS (ultra high performance liquid chromatography coupled to tandem mass spectrometry) method was developed and validated to detect 38 anthelmintic drug residues, consisting of benzimidazoles, avermectins and flukicides. A modified QuEChERS-type extraction method was developed with an added concentration step to detect most of the analytes at <1 mu g kg(-1) levels in milk. Anthelmintic residues were extracted into acetonitrile using magnesium sulphate and sodium chloride to induce liquid-liquid partitioning followed by dispersive solid phase extraction for cleanup. The extract was concentrated into dimethyl sulphoxide, which was used as a keeper to ensure analytes remain in solution. Using rapid polarity switching in electrospray ionisation, a single injection was capable of detecting both positively and negatively charged ions in a 13 min run time. The method was validated at two levels: the unapproved use level and at the maximum residue level (MRL) according to Commission Decision (CD) 2002/657/EC criteria. The decision limit (CC alpha) of the method was in the range of 0.14-1.9 and 11-123 mu g kg(-1) for drugs validated at unapproved and MRL levels, respectively. The performance of the method was successfully verified for benzimidazoles and levamisole by participating in a proficiency study. (C) 2010 Elsevier B.V. All rights reserved.
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