Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 19, Pages 3269-3274Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2010.01.048
Keywords
Oxidative stress; Urine analysis; Biomarkers; Tyrosine; 8-OH-dG; HPLC-EC array
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Oxidative stress has been implicated in various pathologies as well as in environmental pollutant-induced negative health outcomes. In this work we have developed an analytical method to measure oxidative stress markers namely m-, o-tyrosine, 3-chlorotyrosine, 3-nitrotyrosine, and the DNA damage marker 8-hydroxy deoxyguanosine in human urine. The method involves the base hydrolysis of the urine sample followed by solid phase extraction of target analytes using a reverse phase polymeric sorbent column prior to the HPLC-EC analysis. The recovery studies indicated that the overall method recovery for all analytes were >60%. The limit of quantification of all target analytes was <10 nM with a linear range from 2 nM to 150 mu M. The applicability of this method is demonstrated by analyzing the above markers in healthy human urine (n=10) samples. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
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