Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 40, Pages 6214-6229Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.08.010
Keywords
Bi-directional plate-height measurement; Theory of chromatography; Convective dispersion; A term; van Deemter equation
Funding
- National Science Foundation [0442072, 0854151]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0442072, 0854151] Funding Source: National Science Foundation
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An experimental and theoretical study was conducted of the column characterization technique in which plate heights determined using the conventional pulse-response method are compared with those determined using a bi-directional method where an eluite sample is introduced into one end of a chromatographic column and elution occurs at the same end after the flow direction is reversed inside the column. Experiments are presented for a micropellicular HPLC column before and after its performance has been degraded by repeated sample injections, for a low-pressure column containing nonporous glass particles, and for an H PLC column containing particles with 300 angstrom pores. The results obtained are interpreted in terms of several different theories which apply in various Fourier number ranges. It was shown that the transcolumn contribution to convective dispersion in a chromatographic column is largely responsible for the difference observed between conventional and bi-directional plate-height measurements and that a collocation method can be employed to develop a useful analytical expression for this contribution. (C) 2010 Elsevier B.V. All rights reserved.
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