Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 52, Pages 8073-8078Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.10.052
Keywords
Liquid phase microextraction; GC-FID; Fatty acids; Palm oil; Vegetable oils
Funding
- Universiti Sains Malaysia (USM)
Ask authors/readers for more resources
The development of a two phase hollow fiber liquid-phase microextraction technique, followed by gas-chromatography-flame ionization detection (GC-FID) for the profiling of the fatty acids (FAs) (lauric, myristic, palmitic, stearic, palmitoleic, oleic, linoleic, linolenic and arachidic) in vegetable oils is described. Heptadecanoic acid methyl ester was used as the internal standard. The FAs were transesterified to their corresponding methyl esters prior to the extraction. Extraction parameters such as type of extracting solvent, temperature, extraction time, stirring speed and salt addition were studied and optimized. Recommended conditions were extraction solvent, n-tridecane; extraction time, 35 min; extraction temperature, ambient; without addition of salt. Enrichment factors varying from 37 to 115 were achieved. Calibration curves for the nine FAs were well correlated (r(2) > 0.994) within the range of 10-5000 mu g L-1. The limit of detection (signal:noise, 3) was 4.73-13.21 ng L-1. The method was successfully applied to the profiling of the FAs in palm oils (crude, olein, kernel, and carotino cooking oil) and other vegetable oils (soybean, olive, coconut, rice bran and pumpkin). The encouraging enrichments achieved offer an interesting option for the profiling of the minor and major FAs in palm and other vegetable oils. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available